10 Reasons – Why Animal Cloning Fails

    As we know recently Indian scientists have completed a milestone journey by cloning the world’s first buffalo named Samrupa. But, cloning is the most tedious process in animal biotechnology and there are several reasons for its success rate being very less. In the case of Dolly, the first sheep cloned 13 years ago, out of the 272 trials only five were successful and this time during samrupa’s cloning, out of 100s only one has become successful and that new born calf also died within 5 days. 

    Following may be the 10 most important hurdles in the process of cloning

  1. Selection of defective cells

  2. The animal to be cloned must be in a good health condition and disease free. Even the cells/tissues selected should be defect free both at nuclear and cytoplasmic levels.

  3. Genetically defective variety selection

  4. If the host animal has some hereditary defects then chances of inheritance of the same defect into the offspring is more. So, always genetically improved variety of animals should be selected for cloning.

  5. Non expertise in cloning

  6. The scientist team involved in the cloning process must be experts in the cloning process although the success rate for cloning is less than 1 % of all samples taken.

  7. Infection during cloning

  8. There are always chances of bacterial and viral infection to the samples during cloning.

  9. Contamination of the media

  10. After enucleation of the  host cell and fusion with surrogate mother’s egg cells the recombined cells are grown on artificial media upto blastular eight cell structure. The media used here are most susceptible source of contamination.

  11. Instruments used may be source of contamination

  12. Instruments like knives, blades, needles, sonicators , dishes and others must be properly cleaned and sterilized before use.

  13. Maintaining suitable environment

  14. Incubators, laminar air flows and lab environment are itself sources of contamination. So, aseptic condition and level IV safety measures should be practiced during cloning.

  15. Endotoxin, the main culprit

  16. Even after sterilization, the dead Gram negative bacterial cell wall components (called endotoxins) are the main source of infection. These are of nano grams size and lipopolysaccharide in nature and stable in heat and chemical conditions. So, the cloning process and materials must be protected from endotoxins contaminations.

  17. Selection of suitable surrogate mother

  18. The mother whose ovum and Uterus will be used for the growth of foetus should be disease free and genetically improved.

  19. Post birth precaution

  20. Even after the birth of the offspring contamination free environment should be maintained for a few days or even for month. Since newly born organisms are more susceptible to infections. Like in the case of Samrupa, it died due to respiratory infection within five days of her birth.

    Conclusion

    Expertise, proper handling, selection of suitable trait and endotoxins free processing are required for successful cloning. Indian scientists find many more opportunities of such milestones in animal cloning for the benefit of mankind by garnering the benefits of better livestock.

6 thoughts on “10 Reasons – Why Animal Cloning Fails

  • March 23, 2013 at 9:24 am
    Permalink

    This is my first time visit at here and i am actually

    happy to read everthing at one place.

    Reply
    • March 27, 2013 at 5:05 am
      Permalink

      It is nice to know that you find it useful. Please explore other articles of your use.

      Reply
  • May 21, 2013 at 9:51 pm
    Permalink

    It is really a nice and helpful piece of info. I’m satisfied that you just shared this useful info with us. Please keep us informed like this. Thanks for sharing.

    Reply
    • June 19, 2013 at 12:41 am
      Permalink

      You are most welcome. It is good to know that you found it useful.

      Reply

Have an opinion about this post? Let us know what you think with a comment...